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KMID : 1147520200130020100
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Korean Academy of Basic Medicine & Health Science
2020 Volume.13 No. 2 p.100 ~ p.105
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Effects of MMP-8 Production by LPS and TPCK Stimulation in Fetal Blood Cells
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Park Jang-Cheol
Park Sung-Hyo
Lee Min-Woo
Kim Eun-Jae
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Abstract
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The purpose of our research was to elucidate the effect of Lipopolysaccharide (LPS) stimulation on fetal blood cells for the Matrix metalloproteinase-8 (MMP-8) production and the effect of Nuclear factor kappa B (NF-kB) inhibitor, Tosylphenylalanine chloromethyl ketone (TPCK) on this system. Cord blood at birth was obtained during the elective cesarean section term and using LPS (1 μg/mL), stimulated immediately with or without NF-kB inhibitor, TPCK (10 and 20 mM). The levels of MMP-8 were measured with specific immunoassay in the supernatant after 1 through 6 hours. NF-kB activity was measured using Western blotting. LPS stimulation to cord blood significantly raised the level of MMP-8 with the passage of time, while NF-kB inhibitor by TPCK restrained the MMP-8 elevation by LPS stimulation. MMP-8 production by cord blood with LPS stimulation was repressed by NF-kB inhibitor, TPCK. Our data suggests that NF-kB has a regulatory role on MMP-8 production induced by LPS in fetal blood cells.
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KEYWORD
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Inflammation, Matrix metalloproteinase-8 (MMP-8), Tosylphenylalanine chloromethyl ketone (TPCK)
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